What is eDNA?

eDNA, or environmental DNA, refers to genetic material obtained directly from environmental samples (such as water, soil, or air) rather than from an individual organism. eDNA is used to identify species present in an environment by detecting traces of DNA they leave behind, making it a valuable tool for biodiversity monitoring, ecological studies, and conservation efforts.

Why use eDNA service from Kumbe?

There are two primary challenges with the current eDNA services available: 

First, all services are provided outside of Tanzania. This means that either your team must handle the sampling or a team of consultants needs to be flown in for this task, both of which can lead to significant costs and require specialised expertise, as sampling is one of the most complex stages of the process.

Second, the collected samples cannot be processed within Tanzania at present, necessitating their export. This requires obtaining permits, which typically results in delays and additional expenses.

At Kumbe, we offer a solution

Instead of bringing the frog to the lab, we bring the lab to the frog!

Which services does Kumbe offer?

Plan

Based on your specific requirements and objectives, Kumbe's team of experts will develop a tailored (e)DNA monitoring plan. This plan will include the following components:

  • Where to take samples

  • How to take samples

  • When to take samples

  • Which frequency of sampling is required

Sampling

Kumbe offers eDNA samples from soil & sediments, water and air

Our team of local trained experts will implement the established sampling plan with precision. To maintain the highest level of consistency and quality, the sampling process, DNA extraction and amplification will be carried out on-site.

For insects we use traps (e.g. Malaise traps) to collects species.

Analysis

Metagenomics

Analysis of collective genetic material (genomes) from a mixed community of organisms, gathered from eDNA samples. Instead of isolating individual species, metagenomics analyzes all the DNA present in a sample

Metabarcoding

Taxonomic group analysis (or metabarcoding analysis) for one or more specific groups. Possible groups are fish, mammals, bacteria, vertebrates, amphibians, bivalves and invertebrates

Single species detection

With this method (PCR analysis) one or more specific species are detected. The results is a yes/no answer

Sequencing

In our state-of-the-art lab located in Arusha, we utilise advanced sequencing technologies to generate comprehensive datasets that capture the genetic diversity of each sample.

Analysis and reporting

Building on your initial requirements, we will conduct a thorough analysis of the results, delivering comprehensive and insightful reports. These reports will include detailed data interpretations, relevant visualizations, and actionable recommendations tailored to your specific needs, ensuring clarity and informed decision-making